SSCP Analysis by Using High-Sensitive DNA Stain

Rapid identification of Phytophthora ramorum using PCR-SSCP analysis of ribosomal DNA ITS-1.

AIMS The primary objectives of this study were to determine if a single-strand conformation polymorphism (SSCP) analysis can be used for rapid identification of Phytophthora ramorum, an important quarantine plant pathogen worldwide, and to further assess the potential of the SSCP technique as a taxonomic tool for the genus Phytophthora. METHODS AND RESULTS SSCP of ribosomal DNA internal trans...

PCR-SSCP: a simple and sensitive method for detection of mutations in the genomic DNA.

T e c h n i c a l progress in the determination of DNA sequence has resulted in an enormous amount of DNA sequence data, with technical innovations such as the polymerase chain reaction (PCR) accelerating the rate of increase of this data. This combination of data and techniques makes it possible now to exploit sequence differences between individuals. Such studies not only can answer biologica...

Solid phase purification and SSCP analysis of amplified genomic DNA by capillary electrophoresis.

Detection and identification of point mutations in genomic DNA has proven increasingly important in biomedical research. A variety of methods for the analysis of single base substitutions have been proposed among which Single Strand Conformational Polymorphism (SSCP) quickly gained success due to its simplicity. In this work we present an analytical on-line tool which combines the ease of solid...

Sensitive Forensic DNA Analysis

High-throughput asymmetric-PCR SSCP analysis using well-controlled temperature conditions.

Single-stranded conformational polymorphism (SSCP) analysis is useful for mutation detection and polymorphism identification. To realize high-throughput SSCP analysis using a high-intensity electric field, the gel temperature must be well-controlled. We have developed an electrophoretic system having a pair of glass plates with an efficient temperature-controlling apparatus on each side of the ...